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Anti-HIF-1 alpha Antibody (2F748)
🥰Excellent
Catalog No. TMAY-02183
Anti-HIF-1 alpha Antibody (2F748) is a Rabbit antibody targeting HIF-1 alpha. Anti-HIF-1 alpha Antibody (2F748) can be used in ICC/IF.
Anti-HIF-1 alpha Antibody (2F748)
Anti-HIF-1 alpha Antibody (2F748)
🥰Excellent
Catalog No. TMAY-02183
Anti-HIF-1 alpha Antibody (2F748) is a Rabbit antibody targeting HIF-1 alpha. Anti-HIF-1 alpha Antibody (2F748) can be used in ICC/IF.
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-HIF-1 alpha Antibody (2F748) is a Rabbit antibody targeting HIF-1 alpha. Anti-HIF-1 alpha Antibody (2F748) can be used in ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 2F748 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of Human HIF1A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HIF1A monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus and cytoplasm. |
| Application | ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human HIF-1 alpha / HIF1A (rh HIF-1 alpha / HIF1A; TMPY-01709; Q16665-1; Arg575-Asn826). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | HIF-1 alpha, also known as HIF1A, contains 1 basic helix-loop-helix (bHLH) domain, 1 PAC (PAS-associated C-terminal) domain, and 2 PAS (PER-ARNT-SIM) domains. It is one of the two subunits of Hypoxia-inducible factor-1 (HIF1). HIF1 is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). HIF-1 alpha is expressed in most tissues with the highest levels in the kidney and heart. It is overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. HIF-1 alpha functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, it activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1A plays an essential role in embryonic vascularization, tumor angiogenesis, and the pathophysiology of ischemic disease. HIF-1 alpha binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires the recruitment of transcriptional coactivators such as CREBPB and EP300. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human HIF-1 alpha / HIF1A Protein (TMPY-01709) |
| Antigen Species | Human |
| Biology Area | Cancer Drug Targets |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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