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Anti-HGF Polyclonal Antibody
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Catalog No. TMAB-00852
Anti-HGF Polyclonal Antibody is a Rabbit antibody targeting HGF. Anti-HGF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-HGF Polyclonal Antibody
Anti-HGF Polyclonal Antibody
😃Good
Catalog No. TMAB-00852
Anti-HGF Polyclonal Antibody is a Rabbit antibody targeting HGF. Anti-HGF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-HGF Polyclonal Antibody is a Rabbit antibody targeting HGF. Anti-HGF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (HGF) Polyclonal Antibody, Unconjugated (TMAB-00852) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 2. Sample: Lane 1: Rat Placenta tissue lysates Lane 2: Rat Cerebellum tissue lysates Primary: Anti-HGF (TMAB-00852) at 1/200 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54/80 kDa Observed band size: 60 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:200-500; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | HGF is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. HGF is a potent, mesenchymally-derived mitogen for mature parenchymal hepatocytes, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. HGF regulates many physiological processes including proliferation, scattering, morphogenesis and survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. This recombinant human HGF is produced by human cells. (Tag free) Biological activity: The activity was measured by its ability to stimulate the proliferation of the monkey epithelial cell line 4MBr-5. Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. |
| Immunogen | KLH conjugated synthetic peptide: human HGF alpha |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Hormones,Angiogenic growth factors |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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