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Anti-Hexokinase II Antibody (1M510)
😃Good
Catalog No. TMAB-07005
Anti-Hexokinase II Antibody (1M510) is a Mouse antibody targeting Hexokinase II. Anti-Hexokinase II Antibody (1M510) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Hexokinase II Antibody (1M510)
Anti-Hexokinase II Antibody (1M510)
😃Good
Catalog No. TMAB-07005
Anti-Hexokinase II Antibody (1M510) is a Mouse antibody targeting Hexokinase II. Anti-Hexokinase II Antibody (1M510) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Hexokinase II Antibody (1M510) is a Mouse antibody targeting Hexokinase II. Anti-Hexokinase II Antibody (1M510) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG1, k |
| Clone | 1M510 |
| Reactivity | Human |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding. |
| Tissue Specificity | Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose-6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HXK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines. HXK II is involved in the uptake and utilization of glucose by adipose and skeletal tissues. Of the hexokinases, HXK III has the highest affinity for glucose. Glucokinase is expressed in pancreatic beta cells where it functions as a glucose sensor, determining the “set point” for insulin secretion. |
| Immunogen | Recombinant Protein: human HK2 (Hexokinase II) between 1-170 amino acids |
| Antigen Species | Human |
| Gene Name | HK2 |
| Gene ID | |
| Protein Name | Hexokinase-2 |
| Uniprot ID | |
| Function | Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. |
| Molecular Weight | Theoretical: 101 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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