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Anti-HERC6 Polyclonal Antibody (Synonyms: Probable E3 ubiquitin protein ligase HERC6, Potential ubiquitin ligas...)

Catalog No. TMAB-00848 Copy Product Info
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Anti-HERC6 Polyclonal Antibody is a Rabbit antibody targeting HERC6. Anti-HERC6 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.

Anti-HERC6 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-00848
Synonyms Probable E3 ubiquitin protein ligase HERC6, Potential ubiquitin ligase, HERC6, HERC 6, Hect domain and RLD 6, HECT domain and RCC1-like domain-containing protein 6, HECT domain and RCC1 like domain containing protein 6, FLJ20637

Anti-HERC6 Polyclonal Antibody is a Rabbit antibody targeting HERC6. Anti-HERC6 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.

Anti-HERC6 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-HERC6 Polyclonal Antibody is a Rabbit antibody targeting HERC6. Anti-HERC6 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.
SynonymsProbable E3 ubiquitin protein ligase HERC6, Potential ubiquitin ligase, HERC6, HERC 6, Hect domain and RLD 6, HECT domain and RCC1-like domain-containing protein 6, HECT domain and RCC1 like domain containing protein 6, FLJ20637
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Pig,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HERC6) Polyclonal Antibody, Unconjugated (TMAB-00848) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Rat Placenta tissue lysates
Lane 4: Rat Testis tissue lysates
Lane 5: Human HL-60 cell lysates
Lane 6: Human A431 cell lysates
Lane 7: Human MOLT4 cell lysates
Primary: Anti-HERC6 (TMAB-00848) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 135 kDa
verifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm, cytosol.
Tissue SpecificityDetected in brain, heart, placenta and testis.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human HERC6
Antigen Species
Human
Gene Name
HERC6
Gene ID
Protein Name
Probable E3 ubiquitin-protein ligase HERC6
Uniprot ID
Biology Area
Hect E3 Ligase
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity).
Chemical Properties
Molecular WeightTheoretical: 115 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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