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Anti-HER2/ERBB2 Antibody (3F246)
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Catalog No. TMAB-00847
Anti-HER2/ERBB2 Antibody (3F246) is a Mouse antibody targeting HER2/ERBB2. Anti-HER2/ERBB2 Antibody (3F246) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-HER2/ERBB2 Antibody (3F246)
Anti-HER2/ERBB2 Antibody (3F246)
😃Good
Catalog No. TMAB-00847
Anti-HER2/ERBB2 Antibody (3F246) is a Mouse antibody targeting HER2/ERBB2. Anti-HER2/ERBB2 Antibody (3F246) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-HER2/ERBB2 Antibody (3F246) is a Mouse antibody targeting HER2/ERBB2. Anti-HER2/ERBB2 Antibody (3F246) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 3F246 |
| Reactivity | Human |
| Verified Activity | 1. Sample: Lane 1: A431 (Human) Cell Lysate at 30 μg Lane 2: SW480 (Human) Cell Lysate at 30 μg Lane 3: Siha (Human) Cell Lysate at 30 μg Lane 4: A549 (Human) Cell Lysate at 30 μg Primary: Anti-C-erbB-2/HER2 (TMAB-00847) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 185/100-110 kDa Observed band size: 185 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (C-erbB-2/HER2) Monoclonal Antibody, conjugated (TMAB-00847) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 3. Sample: Lane 1: Human A431 cell lysates Lane 2: Human LOVO cell lysates Lane 3: Human HeLa cell lysates Primary: Anti-C-erbB-2/HER2 (TMAB-00847) at 1/200 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 138 kDa Observed band size: 180 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:200-1000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus.Isoform 2: Cytoplasm. Nucleus.Isoform 3: Cytoplasm. Nucleus. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]. |
| Immunogen | Recombinant Protein: human HER2 protein |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | EGF,Oncoproteins,Tumor-associated antigens,EGF,Receptor Tyrosine Kinases,Tumor Associated |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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