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Anti-HE4 Antibody (3F147)
Catalog No. TMAB-06955 Copy Product Info
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Anti-HE4 Antibody (3F147) is a Mouse antibody targeting HE4. Anti-HE4 Antibody (3F147) can be used in IHC-P,IHC-Fr,IF.
Anti-HE4 Antibody (3F147)
Copy Product Info🥰Excellent
Catalog No. TMAB-06955
Anti-HE4 Antibody (3F147) is a Mouse antibody targeting HE4. Anti-HE4 Antibody (3F147) can be used in IHC-P,IHC-Fr,IF.
Anti-HE4 Antibody
(3F147)
(3F147)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 100 μL | $352 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-HE4 Antibody (3F147) is a Mouse antibody targeting HE4. Anti-HE4 Antibody (3F147) can be used in IHC-P,IHC-Fr,IF. |
| Clone | 3F147 |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human Ovarian Tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (HE4) Monoclonal Antibody, Unconjugated (TMAB-06955) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (HE4) Monoclonal Antibody, Unconjugated (TMAB-06955) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HE4 Monoclonal Antibody, Unconjugated (TMAB-06955) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HE4 Monoclonal Antibody, Unconjugated (TMAB-06955) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Endometrial; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HE4 Monoclonal Antibody, Unconjugated (TMAB-06955) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Secreted |
| Tissue Specificity | Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant Protein: human WAP four-disulfide core domain protein 2 |
| Antigen Species | Human |
| Gene Name | WFDC2 |
| Gene ID | |
| Protein Name | WAP four-disulfide core domain protein 2 |
| Uniprot ID | |
| Function | Broad range protease inhibitor. |
| Molecular Weight | Theoretical: 13 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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