Anti-HCN4 Polyclonal Antibody is a Rabbit antibody targeting HCN4. Anti-HCN4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Pig,Cow,Rabbit)

1. Sample:
Lane 1: Mouse Testis Lysates
Lane 2: Rat Testis Lysates
Lane 3: Human HL-60 cell Lysates
Lane 4: Human MCF-7 cell Lysates
Lane 5: Human A549 cell Lysates
Primary: Anti-HCN4 (TMAB-00837) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 129 kDa
Observed band size: 129 kDa
2. Sample:
Heart (Mouse) Lysate at 40 μg
Primary: Anti-HCN4 (TMAB-00837) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 129 kDa
Observed band size: 129 kDa
3. Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-HCN4 Polyclonal Antibody, Unconjugated (TMAB-00837) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Blank control: MCF-7. Primary Antibody (green line): Rabbit Anti-HCN4 antibody (TMAB-00837)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human HCN4

Human

Cardiac arrhythmias,More Channels,More Ion Channels,Taste system