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Anti-HAPLN3 Polyclonal Antibody is a Rabbit antibody targeting HAPLN3. Anti-HAPLN3 Polyclonal Antibody can be used in FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
| Description | Anti-HAPLN3 Polyclonal Antibody is a Rabbit antibody targeting HAPLN3. Anti-HAPLN3 Polyclonal Antibody can be used in FCM. |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Human,Rat,Pig,Horse,Chimpanzee) |
| Verified Activity | Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-HAPLN3 antibody (TMAB-06886) Dilution: 2 μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488 Dilution: 1 μg /test. Protocol The cells were fixed with 70% ethanol (10 min at room temperature) and then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | FCM: 2μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix (By similarity). |
| Tissue Specificity | Widely expressed with highest levels in spleen and placenta. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | HAPLN3 is a 360 amino acid protein encoded by the human gene HAPLN3. HAPLN3 belongs to the HAPLN family and contains 1 Ig-like V-type (immunoglobulin-like) domain and two Link domains. HAPLN3 mediates the binding of complexes containing hyaluronic acid. May play a pivotal role in the formation of the hyaluronan-associated matrix in the central nervous system (CNS) which facilitates neuronal conduction and general structural stabilization. HAPLN3 may also be involved in the formation of extracellular matrices contributing to perineuronal nets and facilitate the understanding of a functional role of these extracellular matrices. HAPLN3 is widely expressed with highest levels in spleen and placenta. |
| Immunogen | KLH conjugated synthetic peptide: human HAPLN3 |
| Antigen Species | Human |
| Gene Name | HAPLN3 |
| Gene ID | |
| Protein Name | Hyaluronan and proteoglycan link protein 3 |
| Uniprot ID | |
| Function | May function in hyaluronic acid binding. |
| Molecular Weight | Theoretical: 39 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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