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Anti-HA Tag Antibody (5Z189)
😃Good
Catalog No. TMAY-00030
Anti-HA Tag Antibody (5Z189) is a Mouse antibody targeting HA Tag. Anti-HA Tag Antibody (5Z189) can be used in IP.
Anti-HA Tag Antibody (5Z189)
Anti-HA Tag Antibody (5Z189)
😃Good
Catalog No. TMAY-00030
Anti-HA Tag Antibody (5Z189) is a Mouse antibody targeting HA Tag. Anti-HA Tag Antibody (5Z189) can be used in IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $94 | 7-10 days | |
| 100 μL | $187 | 7-10 days | |
| 500 μL | $469 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: IP Reactivity: other |
| Ig Type | Mouse IgG1 |
| Clone | 5Z189 |
| Reactivity | other |
| Verified Activity | HA tag was immunoprecipitated using: -Lane A:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate. -Lane B:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate. -2 µL anti-HA tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-HA tag mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 39 kDa. -Observed band size: 39 kDa |
| Application | IP |
| Recommended Dose | IP: 4-8 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic peptide corresponding to the HA-tag sequence. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The human influenza hemagglutinin (HA) epitope was derived from the protein on the HA virus. HA tag is the amino acid sequence of a YPYDVPDYA tag and a particularly common tags. HA is tagged onto proteins for study and analysis and often used in fusion protein-related research through the realization of detection of HA-tag for the detection of fusion proteins. Antibodies to HA-tag are the key of the most important tools in the detection and often used in purification of HA tag-related proteins, study of the protein interaction and detection of target protein expression. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: HA-tag sequence (YPYDVPDYA). |
| Antigen Species | other |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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