Anti-H2-Q10 Polyclonal Antibody is a Rabbit antibody targeting H2-Q10. Anti-H2-Q10 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Mouse

1. Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-H2-Q10 antibody (TMAB-06863)
Dilution: 1 μg/Test;
Secondary Antibody (green line) : Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Negative control (white blue line): PBS
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-H2-Q10 antibody (TMAB-06863)
Dilution: 1 μg/Test;
Secondary Antibody (green line) : Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Negative control (white blue line): PBS
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
3. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (H2-Q10) Polyclonal Antibody, Unconjugated (TMAB-06863) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (H2-Q10) Polyclonal Antibody, Unconjugated (TMAB-06863) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
5. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Testis tissue lysates
Primary: Anti-H2-Q10 (TMAB-06863) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa

WB: 1:500-2000; IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

Recombinant Protein: mouse H2-Q10 protein

Mouse

H2-Q10

H-2 class I histocompatibility antigen, Q10 alpha chain

Involved in the presentation of foreign antigens to the immune system.