Anti-GTPBP9 Polyclonal Antibody is a Rabbit antibody targeting GTPBP9. Anti-GTPBP9 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Cow,Sheep,Fruit Fly)

1. Blank control (Black line): Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-GTPBP9 antibody (TMAB-06854)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
HepG2 Cell (Human) Lysate at 30 μg
TT Cell (Human) Lysate at 30 μg
Primary: Anti-GTPBP9 (TMAB-06854) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD
3. Sample: Raji Cell (Human) Lysate at 30 μg
Primary: Anti-GTPBP9 (TMAB-06854) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD
4. Sample: Brain (Mouse) Lysate at 40 μg
Primary: Anti-GTPBP9 (TMAB-06854) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human GTPBP9

Human

OLA1

Obg-like ATPase 1

Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.