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Anti-GST Tag Antibody (3S373)
😃Good
Catalog No. TMAY-01756
Anti-GST Tag Antibody (3S373) is a Mouse antibody targeting GST Tag. Anti-GST Tag Antibody (3S373) can be used in WB,IP.
Anti-GST Tag Antibody (3S373)
Anti-GST Tag Antibody (3S373)
😃Good
Catalog No. TMAY-01756
Anti-GST Tag Antibody (3S373) is a Mouse antibody targeting GST Tag. Anti-GST Tag Antibody (3S373) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $92 | 7-10 days | |
| 100 μL | $186 | 7-10 days | |
| 500 μL | $469 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-GST Tag Antibody (3S373) is a Mouse antibody targeting GST Tag. Anti-GST Tag Antibody (3S373) can be used in WB,IP. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 3S373 |
| Reactivity | Schistosoma japonicum |
| Verified Activity | 1. Anti-GST Tag was immunoprecipitated using: -Lane A: GST-ARG1-Flag transfected 293 cell lysate (0.5mg) -Lane B: Flag-ARG1-GST transfected 293 cell lysate (0.5mg) -2 μg anti-HA Tag mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-GST Tag rabbit monoclonal antibody, at 1:100 dilutionSecondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 2. Recognize GST tag fusion protein human ERK2.Anti-GST Tag mouse monoclonal antibody at 1:4000 -Lane A: GST-ERK2 (Recombinant protein) (100ng) -Lane B: GST-ERK2 (Recombinant protein) (50ng) -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 3. Recognize GST tag fusion protein human USP7.Anti-GST Tag mouse monoclonal antibody at 1:4000 -Lane A: GST-USP7 (Recombinant protein) (100ng) -Lane B: GST-USP7 (Recombinant protein) (50ng) -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 4. Recognize GST tag fusion proteins which expressed by 293 cell. -Anti-GST Tag mouse monoclonal antibody at 1:1250 or 1:2500 -Lane A: GST-FABP4-flag transfected 293 Cell Lysate (30ug) -Lane B: flag-FABP4-GST transfected 293 Cell Lysate (30ug) -Lane C: flag-AGR1-GST transfected 293 Cell Lysate (30ug) -Lane D: Negative control -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 5. Recognize GST tag fusion proteins which expressed by E. coli.Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250 -Lane A: GST-mBID transfected E.coli cell lysate (0.5ug) -Lane B: GST-mBID transfected E.coli cell lysate (0.2ug) -Lane C: GST-mBID transfected E.coli cell lysate (0.1ug) -Lane D: GST-mBID transfected E.coli cell lysate (0.05ug) -Lane E: GST-mBID transfected E.coli cell lysate (0.02ug) -Lane F: GST-YWHAB transfected E.coli cell lysate (0.5ug) -Lane G: GST-YWHAB transfected E.coli cell lysate (0.2ug) -Lane H: GST-YWHAB transfected E.coli cell lysate (0.1ug) -Lane I: GST-YWHAB transfected E.coli cell lysate (0.05ug) -Lane J: GST-YWHAB transfected E.coli cell lysate (0.02ug) -Lane K: Empty vecter (0.5ug) -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 6. Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:1000 -Lane A: GST-mBID transfected E.coli cell lysate (0.1ug) -Lane B: GST-YWHAB transfected E.coli cell lysate (0.1ug) -Lane C: Empty vecter (0.1ug) -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 7. Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:25000 -Lane A: GST-mBID transfected E.coli cell lysate (0.2ug) -Lane B: GST-YWHAB transfected E.coli cell lysate (0.2ug) -Lane C: Empty vecter (0.2ug) -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. |
| Application | WB,IP |
| Recommended Dose | WB: 1:1000-1:10000; IP: 1-4 μl/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Glutathione S-transferase (GST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The glutathione S-transferase (GST) tag is another well-established affinity tag based on the strong affinity of GST for immobilized glutathione. The GST tag is best suitable for use in prokaryotic expression because GSTs are a family of multifunctional cytosolic proteins that are present in eukaryotic organisms but generally not found in bacteria. Similar to the MBP tag, GST tag has long been used to increase the solubility of fusion proteins in E. coli. GST-tagged proteins are captured by immobilized glutathione and then are eluted under mild, nondenaturing conditions using reduced glutathione. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant GST protein |
| Antigen Species | Schistosoma japonicum |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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