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Anti-GSK3B Antibody (5W152)
😃Good
Catalog No. TMAB-00817
Alias GSK-3β, GSK3β, glycogen synthase kinase 3 β, glycogen synthase kinase 3 beta
Anti-GSK3B Antibody (5W152) is a Mouse antibody targeting GSK3B. Anti-GSK3B Antibody (5W152) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-GSK3B Antibody (5W152)
Anti-GSK3B Antibody (5W152)
😃Good
Catalog No. TMAB-00817Alias GSK-3β, GSK3β, glycogen synthase kinase 3 β, glycogen synthase kinase 3 beta
Anti-GSK3B Antibody (5W152) is a Mouse antibody targeting GSK3B. Anti-GSK3B Antibody (5W152) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-GSK3B Antibody (5W152) is a Mouse antibody targeting GSK3B. Anti-GSK3B Antibody (5W152) can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | GSK-3β, GSK3β, glycogen synthase kinase 3 β, glycogen synthase kinase 3 beta |
| Ig Type | IgG |
| Clone | 5W152 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Hela (Human) Cell Lysate at 30 μg Hela ko GSK-3 Beta (Human) Cell Lysate at 30 μg Primary: Anti-GSK-3 Beta (TMAB-00817) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa 2. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Pancreas (Mouse) Lysate at 40 μg Lane 3: Muscle (Rat) Lysate at 40 μg Lane 4: Cerebrum (Rat) Lysate at 40 μg Lane 5: Hela (Human) Cell Lysate at 30 μg Lane 6: MCF-7 (Human) Cell Lysate at 30 μg Lane 7: A431 (Human) Cell Lysate at 30 μg Lane 8: U2os (Human) Cell Lysate at 30 μg Primary: Anti-GSK-3 Beta (TMAB-00817) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 45 kDa 3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (TMAB-00817) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (TMAB-00817) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:200-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. Nucleus. Cell membrane. Note=The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
| Immunogen | KLH conjugated synthetic peptide: human GSK-3 Beta |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Integration of energy metabolism,Metabolism of carbohydrates,Diabetes associated,Hypertrophy,Carbohydrate metabolism,Integration of energy,Cancer,Diabetes,Heart disease,Notch Pathway,Other Kinases,Cytoplasmic,Cytoplasmic |
| Molecular Weight | Theoretical: 47 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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