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Anti-GRPEL2 Polyclonal Antibody
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Catalog No. TMAB-06804 Copy Product Info
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Anti-GRPEL2 Polyclonal Antibody is a Rabbit antibody targeting GRPEL2. Anti-GRPEL2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-GRPEL2 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 µL | $220 | 7-10 days | 7-10 days | |
| 100 µL | $372 | 7-10 days | 7-10 days | |
| 200 µL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-GRPEL2 Polyclonal Antibody is a Rabbit antibody targeting GRPEL2. Anti-GRPEL2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Rat (predicted:Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GRPEL2) Polyclonal Antibody, Unconjugated (TMAB-06804) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Mitochondrion matrix. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | GrpEL2 (GrpE protein homolog 2) is a 225 amino acid mitochondrial matrix protein and component of the PAM complex. Consisting of Tim44, Tim14, HSP 70, Magmas, GrpEL1 and GrpEL2, the PAM complex plays an essential role in the ATP-dependent translocation of transit peptide-containing proteins to the mitochondrial matrix from the inner membrane. GrpEL2 regulates the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins and stimulates its ATPase activity. The gene encoding GrpEL2 maps to human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. |
| Immunogen | KLH conjugated synthetic peptide: human GRPEL2 |
| Antigen Species | Human |
| Gene Name | GRPEL2 |
| Gene ID | |
| Protein Name | GrpE protein homolog 2, mitochondrial |
| Uniprot ID | |
| Function | Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. Stimulates ATPase activity of mt-HSP70. May also serve to modulate the interconversion of oligomeric (inactive) and monomeric (active) forms of mt-HSP70. |
| Molecular Weight | Theoretical: 21 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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