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Anti-GRP78/Bip Antibody (8X886)
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Catalog No. TMAB-06801
Anti-GRP78/Bip Antibody (8X886) is a Rabbit antibody targeting GRP78/Bip. Anti-GRP78/Bip Antibody (8X886) can be used in IHC-P,IHC-Fr,ICC/IF,IF.
Anti-GRP78/Bip Antibody (8X886)
Anti-GRP78/Bip Antibody (8X886)
Copy Product Info😃Good
Catalog No. TMAB-06801
Anti-GRP78/Bip Antibody (8X886) is a Rabbit antibody targeting GRP78/Bip. Anti-GRP78/Bip Antibody (8X886) can be used in IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $148 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $472 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-GRP78/Bip Antibody (8X886) is a Rabbit antibody targeting GRP78/Bip. Anti-GRP78/Bip Antibody (8X886) can be used in IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| Clone | 8X886 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (GRP78/Bip) monoclonal Antibody, unconjugated (TMAB-06801) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control. 2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GRP78 Monoclonal Antibody, Unconjugated (TMAB-06801) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GRP78 Monoclonal Antibody, Unconjugated (TMAB-06801) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GRP78 Monoclonal Antibody, Unconjugated (TMAB-06801) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GRP78 Monoclonal Antibody, Unconjugated (TMAB-06801) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GRP78 Monoclonal Antibody, Unconjugated (TMAB-06801) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GRP78 Monoclonal Antibody, Unconjugated (TMAB-06801) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GRP78 Monoclonal Antibody, Unconjugated (TMAB-06801) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate. |
| Immunogen | A synthesized peptide: human GRP78 BiP |
| Antigen Species | Human |
| Gene Name | HSPA5 |
| Gene ID | |
| Protein Name | Endoplasmic reticulum chaperone BiP |
| Uniprot ID | |
| Function | Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. |
| Molecular Weight | Theoretical: 72 kDa. Actual: 78 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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