Anti-GRK1 Polyclonal Antibody is a Rabbit antibody targeting GRK1. Anti-GRK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Cow)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-GRK1 Polyclonal Antibody, Unconjugated (TMAB-06777) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-GRK1 Polyclonal Antibody, Unconjugated (TMAB-06777) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Sample:
Lane 1: Human HUVEC cell lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human Jurkat cell lysates
Lane 4: Human Ramos cell lysates
Primary: Anti-GRK1 (TMAB-06777) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human GRK1

Human

GRK1

Rhodopsin kinase GRK1

Phosphorylates rhodopsin thereby initiating its deactivation. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.