Anti-GRIN2A Antibody (2K204) is a Rabbit antibody targeting GRIN2A. Anti-GRIN2A Antibody (2K204) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

2K204

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Human seahorse; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GRIN2A Monoclonal Antibody, Unconjugated (TMAB-06773) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GRIN2A Monoclonal Antibody, Unconjugated (TMAB-06773) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. 25 ug total protein per lane of various lysates (see on figure) probed with GRIN2A monoclonal antibody, unconjugated (TMAB-06773) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GRIN2A Monoclonal Antibody, Unconjugated (TMAB-06773) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, Goat Anti-Rabbit IgG H&L-BF594), DAPI (blue) was used to stain the cell nuclei.

WB: 1:200-1000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200

Monoclonal

A synthesized peptide: human GluN2A

Human

GRIN2A

Glutamate receptor ionotropic, NMDA 2A

NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits (By similarity).