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Anti-GRIN1 Antibody (5R681) is a Rabbit antibody targeting GRIN1. Anti-GRIN1 Antibody (5R681) can be used in IF,IHC-Fr,IHC-P,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $154 | 7-10 days | 7-10 days | |
| 50 μL | $273 | 7-10 days | 7-10 days | |
| 100 μL | $487 | 7-10 days | 7-10 days |
| Description | Anti-GRIN1 Antibody (5R681) is a Rabbit antibody targeting GRIN1. Anti-GRIN1 Antibody (5R681) can be used in IF,IHC-Fr,IHC-P,WB. |
| Synonyms | N-methyl-D-aspartate receptor subunit NR1 (NMD-R1;hNR1), NMDAR1, NMDA 1, GRIN1, Glutamate receptor ionotropic, NMDA 1, Glutamate [NMDA] receptor subunit zeta-1, GluN1 |
| Ig Type | IgG |
| Clone | 5R681 |
| Reactivity | Rat,Mouse,Human |
| Verified Activity | 1. 25 μg total protein per Lane of various lysates probed with NMDAR1 monoclonal antibody, unconjugated (TMAB-00813) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NMDAR1 Monoclonal Antibody, Unconjugated (TMAB-00813) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NMDAR1 Monoclonal Antibody, Unconjugated (TMAB-00813) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NMDAR1 Monoclonal Antibody, Unconjugated (TMAB-00813) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NMDAR1 Monoclonal Antibody, Unconjugated (TMAB-00813) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NMDAR1 Monoclonal Antibody, Unconjugated (TMAB-00813) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. ![]() ![]() ![]() ![]() ![]() ![]() |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Note=Enriched in post-synaptic plasma membrane and post-synaptic densities. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthesized peptide: human GluN1 |
| Antigen Species | Human |
| Gene Name | GRIN1 |
| Gene ID | |
| Protein Name | Glutamate receptor ionotropic, NMDA 1 |
| Uniprot ID | |
| Biology Area | Growth Cone,Neurogenesis |
| Function | NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. |
| Molecular Weight | Theoretical: 103 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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