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Anti-GRIN1 Antibody (2T28) is a Rabbit antibody targeting GRIN1. Anti-GRIN1 Antibody (2T28) can be used in FCM,ICC/IF,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $297 | 7-10 days | 7-10 days | |
| 100 μL | $496 | 7-10 days | 7-10 days |
| Description | Anti-GRIN1 Antibody (2T28) is a Rabbit antibody targeting GRIN1. Anti-GRIN1 Antibody (2T28) can be used in FCM,ICC/IF,IHC,WB. |
| Synonyms | N-methyl-D-aspartate receptor subunit NR1 (NMD-R1, NMDAR1, NMDA 1, hNR1), GRIN1, Glutamate receptor ionotropic, NMDA 1, Glutamate [NMDA] receptor subunit zeta-1, GluN1 |
| Ig Type | IgG |
| Clone | 2T28 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of NMDAR1 on different cells lysates using anti-NMDAR1 antibody at 1/500 dilution. Positive control: Line 1: MCF-7, Line 2: A549. 2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NMDAR1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NMDAR1 antibody. Counter stained with hematoxylin. 4. ICC staining NMDAR1 in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining NMDAR1 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining NMDAR1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of SH-SY5Y cells with NMDAR1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 105 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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