Anti-GRIA2 Polyclonal Antibody is a Rabbit antibody targeting GRIA2. Anti-GRIA2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Dog,Pig)

1. Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GLUR2 Polyclonal Antibody, Unconjugated (TMAB-00811) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Blank control: RSC96 (blue)
Isotype Control Antibody: Rabbit Igg (orange);
Secondary Antibody: Goat anti-rabbit IgG-AF647 (white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA;
Primary Antibody Dilution: 1 μg
L in 100 uL1X PBS containing 0.5% BSA (green).
3. Blank control: RSC96 (blue)
Isotype Control Antibody: Rabbit IgG-AF647 (orange);
Primary Antibody Dilution: 5ul in 100uL1X PBS containing 0.5% BSA (green).
4. Sample: HepG2 Cell Lysate at 30 μg
Primary: Anti-GluR2 (TMAB-00811) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 97 kDa
Observed band size: 117 kDa
5. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GLUR2) Polyclonal Antibody, Unconjugated (TMAB-00811) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GLUR2) Polyclonal Antibody, Unconjugated (TMAB-00811) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
7. Blank control: RSC96 (blue)
Isotype Control Antibody: Rabbit IgG-AF647 (orange); Primary Antibody Dilution: 5ul in 100uL1X PBS containing 0.5% BSA (green).

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg /test

Polyclonal

KLH conjugated synthetic peptide: human GluR2

Human

Glutamate Receptors,Glutamate