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Anti-GRASP65 Antibody (9N637) is a Rabbit antibody targeting GRASP65. Anti-GRASP65 Antibody (9N637) can be used in WB,ICC,IF,IHC,FCM,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $298 | 7-10 days | |
100 μL | $498 | 7-10 days |
Description | Anti-GRASP65 Antibody (9N637) is a Rabbit antibody targeting GRASP65. Anti-GRASP65 Antibody (9N637) can be used in WB,ICC,IF,IHC,FCM,IP. |
Synonyms | P65, MGC118897, MGC118894, GRASP65, GRASP 65, GORS1_HUMAN, GORASP1, Gorasp 1, GOLPH5, GOLPH 5, Golgi reassembly-stacking protein of 65 kDa, Golgi reassembly-stacking protein 1, Golgi reassembly stacking protein of 65 kDa, Golgi reassembly stacking protein 1 65kDa, Golgi reassembly stacking protein 1, Golgi reassembly and stacking protein, 65-kD, Golgi reassembly and stacking protein 65 kDa, Golgi reassembly and stacking protein 1, Golgi phosphoprotein 5, Golgi peripheral membrane protein p65, FLJ23443 |
Ig Type | IgG |
Reactivity | Human |
Verified Activity | 1. Western blot analysis of GRASP65 on different cell lysates using anti-GRASP65 at 1/500 dilution. Positive control: Line 1: THP-1, Line 2: SKBR3. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-GRASP65 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human uterine tissue using anti-GRASP65 antibody. Counter stained with hematoxylin. 4. ICC staining GRASP65 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining GRASP65 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining GRASP65 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of MCF-7 cells with GRASP65 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis (By similarity). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 46 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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