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Anti-GRASP65 Antibody (7Y140)
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Catalog No. TMAB-06760
Anti-GRASP65 Antibody (7Y140) is a Rabbit antibody targeting GRASP65. Anti-GRASP65 Antibody (7Y140) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM.
Anti-GRASP65 Antibody (7Y140)
Anti-GRASP65 Antibody (7Y140)
Copy Product Info😃Good
Catalog No. TMAB-06760
Anti-GRASP65 Antibody (7Y140) is a Rabbit antibody targeting GRASP65. Anti-GRASP65 Antibody (7Y140) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-GRASP65 Antibody (7Y140) is a Rabbit antibody targeting GRASP65. Anti-GRASP65 Antibody (7Y140) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM. |
| Ig Type | IgG |
| Clone | 7Y140 |
| Reactivity | Human |
| Verified Activity | 1. 25 μg total protein per lane of various lysates (see on figure) probed with GRASP65 monoclonal antibody, unconjugated (TMAB-06760) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (GRASP65) monoclonal Antibody, unconjugated (TMAB-06760) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control. 3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GRASP65 Monoclonal Antibody, Unconjugated (TMAB-06760) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GRASP65 Monoclonal Antibody, Unconjugated (TMAB-06760) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GRASP65 Polyclonal Antibody, Unconjugated (TMAB-06760) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; IP: 1:20-50; FCM: 1μg/Test |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Golgi apparatus, cis-Golgi network membrane; |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Key structural protein of the Golgi apparatus.The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon.Acting in concert with GORASP2/GRASP55, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks. |
| Immunogen | A synthesized peptide: human GORASP1 |
| Antigen Species | Human |
| Gene Name | GORASP1 |
| Gene ID | |
| Protein Name | Golgi reassembly-stacking protein 1 |
| Uniprot ID |
| Molecular Weight | Theoretical: 46 kDa. Actual: 65 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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