Anti-Granzyme B Antibody (2I920) is a Mouse antibody targeting Granzyme B. Anti-Granzyme B Antibody (2I920) can be used in IHC-P,IHC-Fr,IF.

IgG

2I920

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Granzyme B Monoclonal Antibody, Unconjugated (TMAB-06754) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Granzyme B Monoclonal Antibody, Unconjugated (TMAB-06754) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Granzyme B Monoclonal Antibody, Unconjugated (TMAB-06754) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Granzyme B Monoclonal Antibody, Unconjugated (TMAB-06754) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Granzyme B Monoclonal Antibody, Unconjugated (TMAB-06754) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Granzyme B Monoclonal Antibody, Unconjugated (TMAB-06754) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Red, BF594), DAPI (blue) was used to stain the cell nuclei.
7. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Granzyme B Monoclonal Antibody, Unconjugated (TMAB-06754) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Red, BF594), DAPI (blue) was used to stain the cell nuclei.

IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:200-1000

Monoclonal

KLH conjugated synthetic peptide: human Granzyme B

Human

GZMB

Granzyme B

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.