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Anti-GPX4 Polyclonal Antibody
😃Good
Catalog No. TMAB-00806
Anti-GPX4 Polyclonal Antibody is a Rabbit antibody targeting GPX4. Anti-GPX4 Polyclonal Antibody can be used in WB.
Anti-GPX4 Polyclonal Antibody
Anti-GPX4 Polyclonal Antibody
😃Good
Catalog No. TMAB-00806
Anti-GPX4 Polyclonal Antibody is a Rabbit antibody targeting GPX4. Anti-GPX4 Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB Reactivity: Human,Mouse,Rat (predicted:Pig,Cow,Sheep) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Pig,Cow,Sheep) |
| Verified Activity | 1. Sample: Kidney (mouse) Lysate at 40 μg Primary: Anti-GPX4 (TMAB-00806) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kDa Observed band size: 20 kDa 2. Sample: Lane 1: Testis (Mouse) Tissue Lysate at 40 μg Lane 2: Liver (Mouse) Tissue Lysate at 40 μg Lane 3: Kidney (Mouse) Tissue Lysate at 40 μg Lane 4: Heart (Mouse) Tissue Lysate at 40 μg Lane 5: Testis (Rat) Tissue Lysate at 40 μg Lane 6: Liver (Rat) Tissue Lysate at 40 μg Lane 7: Kidney (Rat) Tissue Lysate at 40 μg Lane 8: Heart (Rat) Tissue Lysate at 40 μg Lane 9: U937 (Human) Cell Lysate at 30 μg Lane 10: HepG2 (Human) Cell Lysate at 30 μg Lane 11: MCF-7 (Human) Cell Lysate at 30 μg Lane 12: Huvec (Human) Cell Lysate at 30 μg Lane 13: 293T (Human) Cell Lysate at 30 μg Primary: Anti-GPX4 (TMAB-00806) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kDa Observed band size: 20-22 kDa 3. Sample: Testis (Mouse) Lysate at 40 μg Primary: Anti-GPX4 (TMAB-00806) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kDa Observed band size: 19 kDa |
| Application | WB |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site. |
| Immunogen | KLH conjugated synthetic peptide: human Glutathione Peroxidase 4 |
| Antigen Species | Human |
| Gene ID | 2879 |
| Uniprot ID | |
| Biology Area | Cellular metabolic process,Mitochondrial markers,Antioxidants,Cancer,Mitochondrial |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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