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Anti-GPX4 Antibody (5M410) is a Rabbit antibody targeting GPX4. Anti-GPX4 Antibody (5M410) can be used in WB,IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $497 | 7-10 days |
Description | Anti-GPX4 Antibody (5M410) is a Rabbit antibody targeting GPX4. Anti-GPX4 Antibody (5M410) can be used in WB,IHC. |
Synonyms | snPHGPx, snGPx, PHGPx, NEDSGA, MCSP, GSHPx-4, GPx-4, GLURD, GLUR4C, GLUR4, GluA4 |
Ig Type | IgG |
Clone | 5M410 |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of GPX4 on different lysates with GPX4 antibody at 1/10,000 dilution, Lane 1: HeLa cell lysate (20 μg/Lane), Lane 2: A549 cell lysate (20 μg/Lane), Lane Observed band size: 20 kDa, Exposure time: 2 minutes; 4-20% SDS-PAGE gel. 2. Western blot analysis of GPX4 on zebrafish tissue lysates with GPX4 antibody at 1/500 dilution. Lysates/proteins at 10 μg/Lane. Predicted band size: 22 kDa, Observed band size: 17 kDa, Exposure time: 2 minutes; 12% SDS-PAGE gel. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue with GPX4 antibody at 1/1,000 dilution. 4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with GPX4 antibody at 1/1,000 dilution. 5. Immunohistochemical analysis of paraffin-embedded mouse testis tissue with GPX4 antibody at 1/5,000 dilution. |
Application | |
Recommended Dose | WB: 1:10000; IHC-P: 1:1000-5000 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Glutathione peroxidase (GPx) enzymes are generally selenium-containing tetrameric glycoproteins that help prevent lipid peroxidation of cell membranes. GPx enzymes reduce lipid hydroperoxides to alcohols, and reduce free hydrogen peroxide to water. GPx members are among the few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. There are eight GPx homologs (GPx-1-8). GPx-1, Gpx-2 and Gpx-3 exist as homotetramers. Gpx-4 has a high tendancy to form high molecular weight oligomers. |
Conjucates | Unconjugated |
Immunogen | A synthesized peptide: within Human GPX4 |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Theoretical: 22 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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