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Anti-GPX4 Antibody (1B328)
😃Good
Catalog No. TMAB-00807
Anti-GPX4 Antibody (1B328) is a Rabbit antibody targeting GPX4. Anti-GPX4 Antibody (1B328) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-GPX4 Antibody (1B328)
Anti-GPX4 Antibody (1B328)
😃Good
Catalog No. TMAB-00807
Anti-GPX4 Antibody (1B328) is a Rabbit antibody targeting GPX4. Anti-GPX4 Antibody (1B328) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μL | $155 | 7-10 days | |
| 50 μL | $272 | 7-10 days | |
| 100 μL | $487 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: WB,IHC-P,IHC-Fr,ICC/IF,IF Reactivity: Human,Mouse,Rat |
| Ig Type | IgG |
| Clone | 1B328 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. 25 μg total protein per Lane of various lysates probed with GPX4 monoclonal antibody, unconjugated (TMAB-00807) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GPX4 Monoclonal Antibody, Unconjugated (TMAB-00807) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GPX4 Monoclonal Antibody, Unconjugated (TMAB-00807) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GPX4 Monoclonal Antibody, Unconjugated (TMAB-00807) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Thyroid cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GPX4 Monoclonal Antibody, Unconjugated (TMAB-00807) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GPX4 Monoclonal Antibody, Unconjugated (TMAB-00807) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GPX4 Monoclonal Antibody, Unconjugated (TMAB-00807) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GPX4 Monoclonal Antibody, Unconjugated (TMAB-00807) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Mouse testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GPX4 Monoclonal Antibody, Unconjugated (TMAB-00807) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GPX4 Monoclonal Antibody, Unconjugated (TMAB-00807) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Mouse adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GPX4 Monoclonal Antibody, Unconjugated (TMAB-00807) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
| Application | WB,IHC-P,IHC-Fr,ICC/IF,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Research Background | Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. |
| Immunogen | A synthesized peptide: human GPX4 |
| Antigen Species | Human |
| Gene ID | 2879 |
| Uniprot ID | |
| Biology Area | Cellular metabolic process,Mitochondrial markers,Antioxidants,Cancer,Mitochondrial |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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