Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF,FCM,ELISA

Reactivity: Human,Mouse,Rat,Sheep (predicted:Dog,Pig,Cow,Horse,GuineaPig)

IgG

Human,Mouse,Rat,Sheep (predicted:Dog,Pig,Cow,Horse,GuineaPig)

1. Sample: Spleen (Mouse) Lysate at 40 μg
Primary: Anti-GPX1 (TMAB-00805) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
2. Blank control: U937. Primary Antibody (green line): Rabbit Anti-GPX1 antibody (TMAB-00805)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at PBST. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Mouse Kidney tissue lysates
Lane 4: Rat Liver tissue lysates
Lane 5: Rat Heart tissue lysates
Lane 6: Rat Spleen tissue lysates
Lane 7: Rat Kidney tissue lysates
Primary: Anti-GPX1 (TMAB-00805) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa

WB,IHC-P,IHC-Fr,IF,FCM,ELISA

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2ug/Test; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: mouse Glutathione Peroxidase 1

Mouse

14775

Cellular metabolic process,Oxidative Stress,Mitochondrial markers,Antioxidants,Oxidative stress,Cancer,Mitochondrial