Anti-GPR91 Polyclonal Antibody is a Rabbit antibody targeting GPR91. Anti-GPR91 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse

1. Tissue/cell: human breast cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated (TMAB-00801) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Western blot analysis of extracts from Liver tissue (mo) using GPR91 Antibody.
3. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated (TMAB-00801) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Protein: 1.kidney lyates (mo);2.liver lyates (mo);
Primary: Rabbit Anti-GPR91 (TMAB-00801) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit Igg (H+L) at 1: 10000;
Predicted band size:39 kDa
Observed band size:49 kDa
5. Sample: HepG2 Cell (Human) Lysate at 40 μg
Primary: Anti-GPR91 (TMAB-00801) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human GPR91

Human

GPCR,Other Cell Types