Anti-GPR78 Polyclonal Antibody is a Rabbit antibody targeting GPR78. Anti-GPR78 Polyclonal Antibody can be used in WB,FCM.

IgG

Human

1. Blank control: 293T cells (blue). Primary Antibody: Rabbit Anti-GPR78/AF488 Conjugated antibody (TMAB-06729-AF488), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488 (orange), used under the same conditions.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA+1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (TMAB-06729-AF488, 1 μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
2. Blank control (blue line): U937 (blue).
Primary Antibody (green line): Rabbit Anti-GPR7 antibody (TMAB-06729)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
3. Sample: A549 Cell (Human) Lysate at 30 μg
Primary: Anti-GPR78 (TMAB-06729) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human GPR78

Human

GPR78

G-protein coupled receptor 78

Orphan receptor. Displays a significant level of constitutive activity. Its effect is mediated by G(s)-alpha protein that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP.