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Anti-GPR52 Polyclonal Antibody

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Catalog No. TMAB-06722

Anti-GPR52 Polyclonal Antibody is a Rabbit antibody targeting GPR52. Anti-GPR52 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-GPR52 Polyclonal Antibody

Anti-GPR52 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-06722
Anti-GPR52 Polyclonal Antibody is a Rabbit antibody targeting GPR52. Anti-GPR52 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2227-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-GPR52 Polyclonal Antibody is a Rabbit antibody targeting GPR52. Anti-GPR52 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-GPR52 Polyclonal Antibody, Unconjugated (TMAB-06722) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining
2. Sample: A549 Cell Lysate at 40 μg
Primary: Anti-GPR52 (TMAB-06722) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundG protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR52 (G protein-coupled receptor 52) is a 361 amino acid multi-pass membrane protein that belongs to the G-protein coupled receptor 1 family and has been found to be expressed in the caudate and putamen regions of the brain. The gene encoding GPR52 maps to human chromosome 1, the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human GPR52
Antigen Species
Human
Gene Name
GPR52
Gene ID
Protein Name
G-protein coupled receptor 52
Uniprot ID
Function
Orphan receptor.
Chemical Properties
Molecular WeightTheoretical: 41 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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