Anti-GPR43 Polyclonal Antibody is a Rabbit antibody targeting GPR43. Anti-GPR43 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Mouse (predicted:Rat)

1. Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GPR43 Polyclonal Antibody, Unconjugated (TMAB-00795) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (GPR43) polyclonal Antibody, Unconjugated (TMAB-00795) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

IHC-P,IHC-Fr,ICC/IF,IF

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human GPR43

Human

GPCR