Anti-GPR4 Polyclonal Antibody is a Rabbit antibody targeting GPR4. Anti-GPR4 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse

1. Blank control: U937.
Primary Antibody (green line): Rabbit Anti-GPR4 antibody (TMAB-06714)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample: Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-GPR4 (TMAB-06714) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 51 kD
3. Sample: U251 (Human) Cell Lysate at 30 μg
Primary: Anti-GPR4 (TMAB-06714) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 43 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human G protein coupled receptor 4

Human

GPR4

G-protein coupled receptor 4

Proton-sensing G-protein coupled receptor couples to multiple intracellular signaling pathways, including GNAS/cAMP, GNAQ/phospholipase C (PLC), and GNA12/GNA13/Rho pathways (PubMed:12955148, PubMed:17462861, PubMed:20211729, PubMed:22110680).Acidosis-induced GPR4 activation increases paracellular gap formation and permeability of vascular endothelial cells through the GNA12/GNA13/Rho GTPase signaling pathway (PubMed:32058960).In the brain may mediate central respiratory sensitivity to CO2H+ (By similarity).