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Anti-GPR31 Polyclonal Antibody

Catalog No. TMAB-06709 Copy Product Info
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Anti-GPR31 Polyclonal Antibody is a Rabbit antibody targeting GPR31. Anti-GPR31 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-GPR31 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-06709

Anti-GPR31 Polyclonal Antibody is a Rabbit antibody targeting GPR31. Anti-GPR31 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-GPR31 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-GPR31 Polyclonal Antibody is a Rabbit antibody targeting GPR31. Anti-GPR31 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GPR31) Polyclonal Antibody, Unconjugated (TMAB-06709) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Sample: HepG2 (Human) Cell Lysate at 40 μg
Primary: Anti-GPR31 (TMAB-06709) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 36 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Multi pass membrane protein.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundEnables G protein-coupled receptor activity and arachidonic acid binding activity. Involved in G protein-coupled receptor signaling pathway and response to acidic pH. Located in plasma membrane. [provided by Alliance of Genome Resources, Apr 2022]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human G protein coupled receptor 31
Antigen Species
Human
Gene Name
GPR31
Gene ID
Protein Name
12-(S)-hydroxy-5, 8, 10, 14-eicosatetraenoic acid receptor
Uniprot ID
Function
GPCR GPR31 is a member of the G protein coupled receptor (GPCR) family. Members of this protein family contain seven putative transmembrane domains and may mediate signaling processes to the interior of the cell via activation of heterotrimeric G proteins. GPCR GPR31 is located on human chromosome 6q27.
Chemical Properties
Molecular WeightTheoretical: 35 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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