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Anti-GPR25 Polyclonal Antibody

Catalog No. TMAB-06706 Copy Product Info
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Anti-GPR25 Polyclonal Antibody is a Rabbit antibody targeting GPR25. Anti-GPR25 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-GPR25 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-06706

Anti-GPR25 Polyclonal Antibody is a Rabbit antibody targeting GPR25. Anti-GPR25 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-GPR25 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2217-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5287-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-GPR25 Polyclonal Antibody is a Rabbit antibody targeting GPR25. Anti-GPR25 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human
Verified Activity
1. Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-GPR25 Polyclonal Antibody, Unconjugated (TMAB-06706) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: human meningioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-GPR25 Polyclonal Antibody, Unconjugated (TMAB-06706) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-GPR25 Polyclonal Antibody, Unconjugated (TMAB-06706) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundG protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein-coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR25 (G protein-coupled receptor 25) is a 361 amino acid multi-pass membrane protein that belongs to the G protein-coupled receptor family and functions as an orphan receptor, possibly playing a role in signal transduction throughout the cell. The gene encoding GPR25 maps to human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human G protein coupled receptor 25
Antigen Species
Human
Gene Name
GPR25
Gene ID
Protein Name
Probable G-protein coupled receptor 25
Uniprot ID
Function
Orphan receptor.
Chemical Properties
Molecular WeightTheoretical: 39 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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