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Anti-GPNMB Antibody (2N38)

Catalog No. TMAB-06676 Copy Product Info
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Anti-GPNMB Antibody (2N38) is a Mouse antibody targeting GPNMB. Anti-GPNMB Antibody (2N38) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-GPNMB Antibody (2N38)

Copy Product Info
🥰Excellent
Catalog No. TMAB-06676

Anti-GPNMB Antibody (2N38) is a Mouse antibody targeting GPNMB. Anti-GPNMB Antibody (2N38) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-GPNMB Antibody
(2N38)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1487-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4727-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-GPNMB Antibody (2N38) is a Mouse antibody targeting GPNMB. Anti-GPNMB Antibody (2N38) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG1, Kappa
Clone
2N38
Reactivity
Human,Rat
Verified Activity
1. Cell line: HeLa
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-06676
2. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, A375, PC-12, THP-1
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 64-70 kDa
Observed MW: 70 kDa
3. Tissue: Human liver
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-06676
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationMembrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tissue SpecificityNot restricted to the melanocytic lineage.
ConstructionRecombinant Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Antigen Details
Gene Name
GPNMB
Gene ID
Protein Name
Transmembrane glycoprotein NMB
Function
Could be a melanogenic enzyme.
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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