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Anti-GPNMB Antibody (1G705)
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Catalog No. TMAB-06677
Anti-GPNMB Antibody (1G705) is a Rabbit antibody targeting GPNMB. Anti-GPNMB Antibody (1G705) can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-GPNMB Antibody (1G705)
Anti-GPNMB Antibody (1G705)
Copy Product Info😃Good
Catalog No. TMAB-06677
Anti-GPNMB Antibody (1G705) is a Rabbit antibody targeting GPNMB. Anti-GPNMB Antibody (1G705) can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-GPNMB Antibody (1G705) is a Rabbit antibody targeting GPNMB. Anti-GPNMB Antibody (1G705) can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Ig Type | IgG |
| Clone | 1G705 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: A375, 2: B16-F0, 3: EL4.IL-2, 4: HeLa Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 64 kDa Observed MW: 120, 95, 65 kDa 2. Tissue: Mouse small intestine Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Rabbit) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-06677 3. Tissue: Human tonsil Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Rabbit) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-06677 4. Cell line: EL4 Fixative: 4% Paraformaldehyde Permeabilization: 90% Methanol Primary ab dilution: 1:100 Secondary ab: Goat anti Rabbit IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for TMAB-06677 5. Cell line: A375 Fixative: 4% Paraformaldehyde Permeabilization: 90% Methanol Primary ab dilution: 1:100 Secondary ab: Goat anti Rabbit IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for TMAB-06677 |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Tissue Specificity | Not restricted to the melanocytic lineage. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Gene Name | GPNMB |
| Gene ID | |
| Protein Name | Transmembrane glycoprotein NMB |
| Function | Could be a melanogenic enzyme. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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