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Anti-GPI Antibody (6K139)
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Catalog No. TMAC-01673
Anti-GPI Antibody (6K139) is a Rabbit antibody targeting GPI. Anti-GPI Antibody (6K139) can be used in WB,ICC,IF,IHC,FCM.
Anti-GPI Antibody (6K139)
Anti-GPI Antibody (6K139)
😃Good
Catalog No. TMAC-01673
Anti-GPI Antibody (6K139) is a Rabbit antibody targeting GPI. Anti-GPI Antibody (6K139) can be used in WB,ICC,IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-GPI Antibody (6K139) is a Rabbit antibody targeting GPI. Anti-GPI Antibody (6K139) can be used in WB,ICC,IF,IHC,FCM. |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of Glucose 6 phosphate isomerase on A549 and HepG2 cells lysates using anti-Glucose 6 phosphate isomerase antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin. 5. ICC staining Glucose 6 phosphate isomerase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Glucose 6 phosphate isomerase in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of A549 cells with Glucose 6 phosphate isomerase antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Glucose-6-phosphate isomerase (GPI) has many other names, including Phosphohexose isomerase (PHI), Neuroleukin (NLK) and Spermantigen-36 (SA-36). GPI is a cytoplasmic homodimer belonging to the GPI family. It is a neurotrophic factor for spinal and sensory neurons and is involved in glycolysis and gluconeogenesis. Defects or mutations in GPI can cause hereditary nonspherocytic hemolytic anemia (HA), hydrops fetalis, immediate neonatal death and neurological impairment. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: human Glucose 6 phosphate isomerase 300-500aa |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 63 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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