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Anti-GPI Antibody (6K139)

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Catalog No. TMAC-01673 Copy Product Info
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Anti-GPI Antibody (6K139) is a Rabbit antibody targeting GPI. Anti-GPI Antibody (6K139) can be used in FCM,ICC,IF,IHC,WB.

Anti-GPI Antibody
(6K139)

Pack SizePriceUSA StockGlobal StockQuantity
50 µL$2967-10 days7-10 days
100 µL$4967-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-GPI Antibody (6K139) is a Rabbit antibody targeting GPI. Anti-GPI Antibody (6K139) can be used in FCM,ICC,IF,IHC,WB.
Ig Type
IgG
Clone
6K139
Reactivity
Human
Verified Activity
1. Western blot analysis of Glucose 6 phosphate isomerase on A549 and HepG2 cells lysates using anti-Glucose 6 phosphate isomerase antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.
5. ICC staining Glucose 6 phosphate isomerase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Glucose 6 phosphate isomerase in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of A549 cells with Glucose 6 phosphate isomerase antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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Application
Recommended Dose
WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundGlucose-6-phosphate isomerase (GPI) has many other names, including Phosphohexose isomerase (PHI), Neuroleukin (NLK) and Spermantigen-36 (SA-36). GPI is a cytoplasmic homodimer belonging to the GPI family. It is a neurotrophic factor for spinal and sensory neurons and is involved in glycolysis and gluconeogenesis. Defects or mutations in GPI can cause hereditary nonspherocytic hemolytic anemia (HA), hydrops fetalis, immediate neonatal death and neurological impairment.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: human Glucose 6 phosphate isomerase 300-500aa
Antigen Species
Human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 63 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: Anti-GPI Antibody (6K139) molecular weight