Anti-GP1BA Antibody (1V805) is a Rabbit antibody targeting GP1BA. Anti-GP1BA Antibody (1V805) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

1V805

Human,Mouse,Rat

1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1: 2000
Primary ab incubation condition: 2 hours at room temperature
Lysate: Human platelets
Protein loading quantity: 20 μg
Exposure time: 30s
Predicted MW: 72 kDa
Observed MW: 120 kDa
2. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GP1BA Monoclonal Antibody, Unconjugated (TMAB-06648) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GP1BA Monoclonal Antibody, Unconjugated (TMAB-06648) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GP1BA Monoclonal Antibody, Unconjugated (TMAB-06648) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human GP1BA/CD42b

Human

GP1BA

Platelet glycoprotein Ib alpha chain

GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.