Anti-GOT1 Antibody (1G203) is a Rabbit antibody targeting GOT1. Anti-GOT1 Antibody (1G203) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

1G203

Human,Rat (predicted:Mouse)

1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: Mouse heart, 3: Rat heart
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 46 kDa
Observed MW: 46 kDa
2. Tissue: Human cerebrum
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Rabbit)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-06642
3. Tissue: Rat kidney
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Rabbit)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-06642

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

GOT1

Aspartate aminotransferase, cytoplasmic

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.