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Anti-GOLPH2/GOLM1 Antibody (3T18)

😃Good
Catalog No. TMAY-02455
Alias PSEC0257, HEL46, GP73, GOLPH2, golgi membrane protein 1, C9orf155, bA379P1.3

Anti-GOLPH2/GOLM1 Antibody (3T18) is a Mouse antibody targeting GOLPH2/GOLM1. Anti-GOLPH2/GOLM1 Antibody (3T18) can be used in IHC-P,ICC/IF.

Anti-GOLPH2/GOLM1 Antibody (3T18)

Anti-GOLPH2/GOLM1 Antibody (3T18)

😃Good
Catalog No. TMAY-02455Alias PSEC0257, HEL46, GP73, GOLPH2, golgi membrane protein 1, C9orf155, bA379P1.3
Anti-GOLPH2/GOLM1 Antibody (3T18) is a Mouse antibody targeting GOLPH2/GOLM1. Anti-GOLPH2/GOLM1 Antibody (3T18) can be used in IHC-P,ICC/IF.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Mouse Monoclonal

Application: IHC-P, ICC/IF

Reactivity: Human
AliasPSEC0257, HEL46, GP73, GOLPH2, golgi membrane protein 1, C9orf155, bA379P1.3
Ig Type
Mouse IgG2a
Clone
3T18
Reactivity
Human
Verified Activity
1. Immunochemical staining of human GP73 in human uterus with mouse monoclonal antibody (8 µg/mL, formalin-fixed paraffin embedded sections).
2. Immunochemical staining of human GP73 in human stomach with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).
3. Immunochemical staining of human GP73 in human liver (cirrhosis) with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections).
4. Immunofluorescence staining of GP73 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human GP73 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to golgi apparatus .
Application
IHC-P,ICC/IF
Recommended Dose
IHC-P: 5-20 μg/mL; ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis product is a recombinant monoclonal antibody expressed from HEK293 cells.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundGolgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2, and GOLM1, is a protein that belongs to the GOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in the colon, prostate, trachea, and stomach. It is expressed at a lower level in testis, muscle, lymphoid tissues, white blood cells, and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at a low level in the normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncytial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human GOLPH2 / GOLM1 Protein
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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