Anti-GNB2 Antibody (1C38) is a Rabbit antibody targeting GNB2. Anti-GNB2 Antibody (1C38) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM.

IgG

1C38

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GNB2 Monoclonal Antibody, Unconjugated (TMAB-06609) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GNB2 Monoclonal Antibody, Unconjugated (TMAB-06609) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GNB2 Monoclonal Antibody, Unconjugated (TMAB-06609) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. 25 μg total protein per lane of various lysates (see on figure) probed with GNB2 monoclonal antibody, unconjugated (TMAB-06609) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
5. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-GNB2 antibody (TMAB-06609, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; IP: 1:20-50; FCM: 1:50-100

Monoclonal

A synthesized peptide: human GNB2

Human

GNB2

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.