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Anti-GNAT3 Polyclonal Antibody
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Catalog No. TMAB-00783
Alias Gustducin alpha 3 chain, Gustducin alpha 3, Guanine nucleotide-binding protein G(t) subunit alpha-3, Guanine nucleotide binding protein alpha transducing 3, Gtn, Gnat 3, Ggust, GDCA
Anti-GNAT3 Polyclonal Antibody is a Rabbit antibody targeting GNAT3. Anti-GNAT3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-GNAT3 Polyclonal Antibody
Anti-GNAT3 Polyclonal Antibody
😃Good
Catalog No. TMAB-00783Alias Gustducin alpha 3 chain, Gustducin alpha 3, Guanine nucleotide-binding protein G(t) subunit alpha-3, Guanine nucleotide binding protein alpha transducing 3, Gtn, Gnat 3, Ggust, GDCA
Anti-GNAT3 Polyclonal Antibody is a Rabbit antibody targeting GNAT3. Anti-GNAT3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-GNAT3 Polyclonal Antibody is a Rabbit antibody targeting GNAT3. Anti-GNAT3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Gustducin alpha 3 chain, Gustducin alpha 3, Guanine nucleotide-binding protein G(t) subunit alpha-3, Guanine nucleotide binding protein alpha transducing 3, Gtn, Gnat 3, Ggust, GDCA |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-GNAT3 Polyclonal Antibody, Unconjugated (TMAB-00783) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GNAT3) Polyclonal Antibody, Unconjugated (TMAB-00783) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Sample: A549 Cell Lysate at 40 μg Primary: Anti-GNAT3 (TMAB-00783) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa 4. Sample: Testis (Mouse) Lysate at 40 μg Primary: Anti-GNAT3 (TMAB-00783) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Dual ditribution pattern; plasmalemmal pattern with apical region localization and cytosolic pattern with localization throughout the cytoplasm. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (e.g., adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, beta and gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their alpha subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Ga subunits have been identified; these include Gs, Gi, Gq and Ga 12/13. Gustducin has been identified as a taste-cell-specific G protein within the Gi subclass of Ga subunit proteins that is most closely related to the transducins and exclusively expressed in taste buds. |
| Immunogen | KLH conjugated synthetic peptide: human GNAT3 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | More GPCR,Taste system,GPCR |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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