Anti-GNA13 Antibody (3C703) is a Mouse antibody targeting GNA13. Anti-GNA13 Antibody (3C703) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG1, Kappa

3C703

Human (predicted:Mouse,Rat)

1. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: HEK-293, 2: HepG2, 3: Rat brain, 4: Neuro-2a
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 44 kDa
Observed MW: 44 kDa
2. Tissue: Human testis
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-06600
3. Cell line: HepG2
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-06600

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500

Monoclonal

GNA13

Guanine nucleotide-binding protein subunit alpha-13

Guanine nucleotide-binding proteins (G proteins) are modulators or transducers in various transmembrane signaling systems. They are composed of 3 subunits; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.