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Anti-GMF beta Polyclonal Antibody
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Catalog No. TMAB-06599
Anti-GMF beta Polyclonal Antibody is a Rabbit antibody targeting GMF beta. Anti-GMF beta Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-GMF beta Polyclonal Antibody
Anti-GMF beta Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-06599
Anti-GMF beta Polyclonal Antibody is a Rabbit antibody targeting GMF beta. Anti-GMF beta Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-GMF beta Polyclonal Antibody is a Rabbit antibody targeting GMF beta. Anti-GMF beta Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Dog,Pig,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GMF beta) Polyclonal Antibody, Unconjugated (TMAB-06599) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GMF beta) Polyclonal Antibody, Unconjugated (TMAB-06599) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-GMF beta (TMAB-06599) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 19 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Glia maturation factor β (GMF-β) belongs to the GMF subfamily of the larger actin-binding protein ADF family. This protein, which is phosphorylated following phorbol ester stimulation, is important for the nervous system. It causes brain cell differentiation, stimulates neural regeneration and inhibits tumor cell proliferation. Overexpression of GMF in astrocytes has been shown to enhance brain-derived neurotrohic factor (BDNF) production. GMF expression is increased by exercise, and the protein is crucial for exercise-induction of BDNF. Through BDNF production, GMF appears to play a role in neuroprotection. In thymoma, T-cell development is maintained by GMF-β being produced by the tumor cells. |
| Immunogen | KLH conjugated synthetic peptide: human GMF beta |
| Antigen Species | Human |
| Gene Name | GMFB |
| Gene ID | |
| Protein Name | Glia maturation factor beta |
| Uniprot ID | |
| Function | This protein causes differentiation of brain cells, stimulation of neural regeneration, and inhibition of proliferation of tumor cells. |
| Molecular Weight | Theoretical: 17 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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