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Anti-GlyR alpha 1+2+3 Polyclonal Antibody
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Catalog No. TMAB-06587
Anti-GlyR alpha 1+2+3 Polyclonal Antibody is a Rabbit antibody targeting GlyR alpha 1+2+3. Anti-GlyR alpha 1+2+3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-GlyR alpha 1+2+3 Polyclonal Antibody
Anti-GlyR alpha 1+2+3 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-06587
Anti-GlyR alpha 1+2+3 Polyclonal Antibody is a Rabbit antibody targeting GlyR alpha 1+2+3. Anti-GlyR alpha 1+2+3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-GlyR alpha 1+2+3 Polyclonal Antibody is a Rabbit antibody targeting GlyR alpha 1+2+3. Anti-GlyR alpha 1+2+3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-GlyR alpha 1/2/3 Polyclonal Antibody, Unconjugated (TMAB-06587) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Sample: Spinal cord (Mouse) Lysate at 40 μg Spinal cord (Rat) Lysate at 40 μg Primary: Anti-GlyR alpha 1+2+3 (TMAB-06587) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 54 kD 3. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Cerebellum tissue lysates Lane 3: Mouse Testis tissue lysates Lane 4: Mouse Lung tissue lysates Lane 5: Rat Cerebrum tissue lysates Lane 6: Rat Cerebellum tissue lysates Lane 7: Rat Testis tissue lysates Lane 8: Human HeLa cell lysates Lane 9: Human A431 cell lysates Lane 10: Human SH-SY5Y cell lysates Primary: Anti-GlyR alpha 1+2+3 (TMAB-06587) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 53 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Glycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission. The receptors are pentameric membrane proteins which form chloride channels. Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation. Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain. |
| Immunogen | KLH conjugated synthetic peptide: human GlyR alpha 1 |
| Antigen Species | Human |
| Gene Name | GLRA1 |
| Gene ID | |
| Protein Name | Glycine receptor subunit alpha-1 |
| Uniprot ID | |
| Function | The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing). |
| Molecular Weight | Theoretical: 49 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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