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Anti-Glycophorin C/CD236 Antibody (7G950)

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Catalog No. TMAB-06576

Anti-Glycophorin C/CD236 Antibody (7G950) is a Rabbit antibody targeting Glycophorin C/CD236. Anti-Glycophorin C/CD236 Antibody (7G950) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM.

Anti-Glycophorin C/CD236 Antibody (7G950)

Anti-Glycophorin C/CD236 Antibody (7G950)

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Catalog No. TMAB-06576
Anti-Glycophorin C/CD236 Antibody (7G950) is a Rabbit antibody targeting Glycophorin C/CD236. Anti-Glycophorin C/CD236 Antibody (7G950) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1487-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4707-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Glycophorin C/CD236 Antibody (7G950) is a Rabbit antibody targeting Glycophorin C/CD236. Anti-Glycophorin C/CD236 Antibody (7G950) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM.
Ig Type
IgG
Clone
7G950
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glycophorin C/CD236 Monoclonal Antibody, Unconjugated (TMAB-06576) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glycophorin C/CD236 Monoclonal Antibody, Unconjugated (TMAB-06576) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glycophorin C/CD236 Monoclonal Antibody, Unconjugated (TMAB-06576) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. 25 μg total protein per lane of various lysates (see on figure) probed with Glycophorin C/CD236 monoclonal antibody, unconjugated (TMAB-06576) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; IP: 1:20-50; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Single-pass type III membrane protein. Note=Linked to the membrane via band 4.1.
Tissue SpecificityGlycophorin-C is expressed in erythrocytes. Glycophorin-D is ubiquitous.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundThis protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells.
Antigen Details
Immunogen
A synthesized peptide: human Glycophorin C
Antigen Species
Human
Gene Name
GYPC
Gene ID
Protein Name
Glycophorin-C
Uniprot ID
Function
This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells.
Chemical Properties
Molecular WeightTheoretical: 14 kDa. Actual: 39 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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