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Anti-Glycophorin A Antibody (4S112)

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Catalog No. TMAB-06575

Anti-Glycophorin A Antibody (4S112) is a Rabbit antibody targeting Glycophorin A. Anti-Glycophorin A Antibody (4S112) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Glycophorin A Antibody (4S112)

Anti-Glycophorin A Antibody (4S112)

Copy Product Info
😃Good
Catalog No. TMAB-06575
Anti-Glycophorin A Antibody (4S112) is a Rabbit antibody targeting Glycophorin A. Anti-Glycophorin A Antibody (4S112) can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1507-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4717-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Glycophorin A Antibody (4S112) is a Rabbit antibody targeting Glycophorin A. Anti-Glycophorin A Antibody (4S112) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Clone
4S112
Reactivity
Human
Verified Activity
1. Western blot analysis of human fetal liver lysate. Using Glycophorin A (TMAB-06575) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Thyroid tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glycophorin A Monoclonal Antibody, Unconjugated (TMAB-06575) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glycophorin A Monoclonal Antibody, Unconjugated (TMAB-06575) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glycophorin A Monoclonal Antibody, Unconjugated (TMAB-06575) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glycophorin A Monoclonal Antibody, Unconjugated (TMAB-06575) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glycophorin A Monoclonal Antibody, Unconjugated (TMAB-06575) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glycophorin A Monoclonal Antibody, Unconjugated (TMAB-06575) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Single-pass type I membrane protein. Note=Appears to be colocalized with SLC4A1.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundComponent of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane (PubMed:35835865). Glycophorin A is the major intrinsic membrane protein of the erythrocyte.
Antigen Details
Immunogen
A synthesized peptide: human Glycophorin A
Antigen Species
Human
Gene Name
GYPA
Gene ID
Protein Name
Glycophorin-A
Uniprot ID
Function
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
Chemical Properties
Molecular WeightTheoretical: 16 kDa. Actual: 38 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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