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Anti-Glutathione Reductase Polyclonal Antibody
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Catalog No. TMAB-06564
Anti-Glutathione Reductase Polyclonal Antibody is a Rabbit antibody targeting Glutathione Reductase. Anti-Glutathione Reductase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Glutathione Reductase Polyclonal Antibody
Anti-Glutathione Reductase Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-06564
Anti-Glutathione Reductase Polyclonal Antibody is a Rabbit antibody targeting Glutathione Reductase. Anti-Glutathione Reductase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Glutathione Reductase Polyclonal Antibody is a Rabbit antibody targeting Glutathione Reductase. Anti-Glutathione Reductase Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow) |
| Verified Activity | 1. Sample: A549 (Human) Cell Lysate at 30 μg SP2/0 (Mouse) Cell Lysate at 30 μg Primary: Anti-Glutathione Reductase (TMAB-06564) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD 2. Paraformaldehyde-fixed, paraffin embedded (Human Pulp); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Glutathione Reductase) Polyclonal Antibody, Unconjugated (TMAB-06564) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Glutathione Reductase) Polyclonal Antibody, Unconjugated (TMAB-06564) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | bs-0837P is one synthetic peptide derived from human Glutathione Reductase. Glutathione reductase (GR) is a member of pyridine nucleotide- disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+?2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis. |
| Immunogen | KLH conjugated synthetic peptide: human Glutathione Reductase |
| Antigen Species | Human |
| Gene Name | GSR |
| Gene ID | |
| Protein Name | Glutathione reductase, mitochondrial |
| Uniprot ID | |
| Function | Maintains high levels of reduced glutathione in the cytosol. |
| Molecular Weight | Theoretical: 57 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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