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Anti-GLUT2 Antibody (4Y169)

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Catalog No. TMAB-06550

Anti-GLUT2 Antibody (4Y169) is a Rabbit antibody targeting GLUT2. Anti-GLUT2 Antibody (4Y169) can be used in WB,FCM.

Anti-GLUT2 Antibody (4Y169)

Anti-GLUT2 Antibody (4Y169)

Copy Product Info
😃Good
Catalog No. TMAB-06550
Anti-GLUT2 Antibody (4Y169) is a Rabbit antibody targeting GLUT2. Anti-GLUT2 Antibody (4Y169) can be used in WB,FCM.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1487-10 days7-10 days
50 μL$2647-10 days7-10 days
100 μL$4707-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-GLUT2 Antibody (4Y169) is a Rabbit antibody targeting GLUT2. Anti-GLUT2 Antibody (4Y169) can be used in WB,FCM.
Ig Type
IgG
Clone
4Y169
Reactivity
Human,Mouse,Rat
Verified Activity
1. 25 μg total protein per lane of various lysates (see on figure) probed with GLUT2 monoclonal antibody, unconjugated (TMAB-06550) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 25 μg total protein per lane of various lysates (see on figure) probed with GLUT2 monoclonal antibody, unconjugated (TMAB-06550) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
3. The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-GLUT2 antibody (TMAB-06550): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
Application
Recommended Dose
WB: 1:500-2000; FCM: 1μg/Test
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Multi-pass membrane protein.
Tissue SpecificityLiver, insulin-producing beta cell, small intestine and kidney.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundFacilitative hexose transporter that mediates the transport of glucose, fructose and galactose. Likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell.
Antigen Details
Immunogen
A synthesized peptide: human GLUT2
Antigen Species
Human
Gene Name
SLC2A2
Gene ID
Protein Name
Solute carrier family 2, facilitated glucose transporter member 2
Uniprot ID
Function
Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.
Chemical Properties
Molecular WeightTheoretical: 57 kDa. Actual: 57 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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