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Anti-Glucose 6 phosphate isomerase Antibody (5T997)

Catalog No. TMAB-06540 Copy Product Info
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Anti-Glucose 6 phosphate isomerase Antibody (5T997) is a Rabbit antibody targeting Glucose 6 phosphate isomerase. Anti-Glucose 6 phosphate isomerase Antibody (5T997) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Glucose 6 phosphate isomerase Antibody (5T997)

Copy Product Info
🥰Excellent
Catalog No. TMAB-06540

Anti-Glucose 6 phosphate isomerase Antibody (5T997) is a Rabbit antibody targeting Glucose 6 phosphate isomerase. Anti-Glucose 6 phosphate isomerase Antibody (5T997) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Glucose 6 phosphate isomerase Antibody
(5T997)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1497-10 days7-10 days
50 μL$2647-10 days7-10 days
100 μL$4707-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Glucose 6 phosphate isomerase Antibody (5T997) is a Rabbit antibody targeting Glucose 6 phosphate isomerase. Anti-Glucose 6 phosphate isomerase Antibody (5T997) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Clone
5T997
Reactivity
Human
Verified Activity
1. 25 μg total protein per lane of various lysates (see on figure) probed with Glucose 6 phosphate isomerase monoclonal antibody, unconjugated (TMAB-06540) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated (TMAB-06540) at 1: 250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated (TMAB-06540) at 1: 250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated (TMAB-06540) at 1: 250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated (TMAB-06540) at 1: 250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated (TMAB-06540) at 1: 250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Glucose 6 phosphate isomerase Monoclonal Antibody, Unconjugated (TMAB-06540) at 1: 250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Secreted.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
A synthesized peptide: human GPI
Gene Name
GPI
Gene ID
Protein Name
Glucose-6-phosphate isomerase
Function
Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
Chemical Properties
Molecular WeightTheoretical: 63 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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